EDUCATION AND EMPLOYMENT

School of Chemistry and Chemical Engineering, Hubei University, B.S., 2009-2013

College of Chemistry and Molecular Sciences, Wuhan University, Ph.D., 2013-2018, supervisor Dr. Xiang Zhou

College of Chemistry and Molecular Sciences, Wuhan University, Postdoc, 2018-2022, supervisor Dr. Xiang Zhou and Dr. Baoliang Song

School of Public Health, Wuhan University, Distinguished Associate Research Fellow, 2022-

RESEARCH FIELD

Long engaged in the research of nucleic acid chemical biology. Based on the identification of nucleic acids by chemical probes, We develop detection techniques for epigenetic modification of nucleic acids, find the relationship between nucleic acid modification and major diseases, and explore the pathogenic mechanism of diseases

PROJECTS

1. Host Youth Fund Project: Studies on the mapping of 5-aldehyde cytosine profiles in transcriptomes based on single-base resolution of chemical markers, the National Natural Science Foundation of China, 21907077, 2020-2022

2. Host Postdoctoral Innovation Talent Support Program, Ministry of Human Resources and Society Security of China, BX201800225, 2018-2020

3. Host General Program of China Postdoctoral Science Foundation: Study on single base resolution detection technology of modified base - uracil in DNA, 2019M652691, 2019-2020

4. Host Postdoctoral Science and Technology Project of Hubei province: Development of new techniques and methods for 5-uronopyrimidine detection and their application in disease diagnosis, 2018-2020

AWARDS AND HONORS

1. Excellent Wall Newspaper Award in the 32nd Academic Annual Conference, Chinese Chemical Society

2. Excellent Wall Newspaper Award in the 31nd Academic Annual Conference, Chinese Chemical Society

3. Postdoctoral Innovation Talent Support Program, 2018

PUBLICATIONS

1.Yafen Wang* and Xiang Zhou, N6-methyladenosine and Its Implications in Viruses, Genomics, Proteomics & Bioinformatics, 2022, in press.

2.Yafen Wang, Wei Yang and Xiang Zhou*, Chemical labelling for m6A detection: opportunities and challenges, Fundamental Research, 2022, 2 (1), 56−58.

3.Yafen Wang#, Zonggui Chen#, Xiong Zhang#, Xiaocheng Weng#, Jikai Deng#,Wei Yang, Fan Wu, Shaoqing Han, Chao Xia, Yu Zhou, Yu Chen and Xiang Zhou*, Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAs, ACS Chemical Biology, 2022, 17 (1), 77−84. (Front Cover)

4.Yafen Wang#, Shaoqing Han#, Ruoxi Ran#, Anling Li#, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Xiaocheng Weng*, Songmei Liu* and Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome, Nature Communications, 2021, 12:5629. (Editor’s Highlights)

5.Yafen Wang#, Xiong Zhang#, Shaoqing Han#, Wei Yang, Zonggui Chen, Fan Wu, Jizhou Liu, Xiaocheng Weng* and Xiang Zhou*, Base-Resolution Analysis of Deoxyuridine at Genome Scale Based on Artificial Incorporation Modified Nucleobase, ACS Central Science, 2021, 7 (6), 973−979. (Front Cover)

6.Yafen Wang, Xiong Zhang, Hui Liu and Xiang Zhou*, Chemical Methods and Advanced Sequencing Technologies for Deciphering mRNA Modifications, Chemical Society Reviews, 2021, 50 (24), 13481–13497.

7.Jizhou Liu#, Wei Yang#, Xiong Zhang, Yafen Wang#* and Xiang Zhou*, Bisulfite-free and quantitative detection of 5-formylcytosine in DNA through qPCR, Chemical Communications, 2021, 57 (100), 13796–13798.

8.Wei Yang#, Shaoqing Han#, Xiong Zhang#, Yafen Wang#, Guangrong Zou, Chaoxing Liu, Muxin Xu, and Xiang Zhou*, Sequencing 5-formyluracil in genomic DNA at single-base resolution, Analytical Chemistry, 2021, 93 (46), 15445−15451.

9.Hongmei Yang#, Yafen Wang#, Jing Tang, Fang Wang* and Zilin Chen*, End-labeling-based electrochemical strategy for detection of adenine methylation in nucleic acid by differential pulse voltammetry, Microchimica Acta, 2021, 188 (8):250.

10.Yafen Wang, Xiong Zhang, Guangrong Zou, Shuang Peng, Chaoxing Liu and Xiang Zhou*, Detection and Application of 5-Formylcytosine and 5-Formyluracil in DNA, Accounts of Chemical Research, 2019, 52 (4), 1016−1024. (Supplementary Cover)

11.Yafen Wang#, Xiong Zhang#, Fan Wu, Zonggui Chen and Xiang Zhou*, Bisulfite-free, single base-resolution analysis of 5-hydroxymethylcytosine in genomic DNA by chemical-mediated mismatch, Chemical Science, 2019, 10 (2), 447−452.

12.Xiong Zhang #, Yafen Wang # and Xiang Zhou*, A Ligation-Based qPCR Amplification Assay for the Radiolabeling-Free Detection of ATP/NAD+ with High Selectivity and Sensitivity, Analytical Chemistry, 2019, 91 (2),1665−1670.

13.Yafen Wang #, Chaoxing Liu #, Fan Wu #, Xiong Zhang, Sheng Liu, Zonggui Chen, Weiwu Zeng, Wei Yang, Xiaolian Zhang, Yu Zhou, Xiaocheng Weng, Zhiguo Wu and Xiang Zhou*, Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe, iScience, 2018, 9, 423−432.

14.Yafen Wang#, Chaoxing Liu#, Xiong Zhang#, Wei Yang, Fan Wu, Guangrong Zou, Xiaocheng Weng* and Xiang Zhou*, Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction, Chemical Science, 2018, 9 (15), 3723−3728.

15.Yafen Wang #, Chaoxing Liu #, Wei Yang #, Guangrong Zou, Xiong Zhang, Fan Wu, Shuyi Yu, Xiaomeng Luo and Xiang Zhou*, Naphthalimide derivatives as multifunctional molecules for detecting 5-formylpyrimidine by both PAGE analysis and dot-blot assays, Chemical Communications, 2018, 54 (12), 1497−1500.

16.Chaoxing Liu #, Yafen Wang #, Wei Yang, Fan Wu, Weiwu Zeng, Zonggui Chen, Jinguo Huang, Guangrong Zou, Xiong Zhang, Shaoru Wang, Xiaocheng Weng, Zhiguo Wu, Yu Zhou and Xiang Zhou*, Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA, Chemical Science, 2017, 8 (11), 7443−7447.

17.Chaoxing Liu #, Yafen Wang #, Xiong Zhang, Fan Wu, Wei Yang, Guangrong Zou, Qian Yao, Jiaqi Wang, Yuqi Chen, Shaoru Wang and Xiang Zhou*, Enrichment and fluorogenic labelling of 5-formyluracil in DNA, Chemical Science, 2017, 8 (6), 4505−4510.

18.Chaoxing Liu #, Yuqi Chen #, Yafen Wang #, Fan Wu, Xiong Zhang, Wei Yang, Jiaqi Wang, Yi Chen, Zhiyong He, Guangrong Zou, Shaoru Wang, and Xiang Zhou*, A highly efficient fluorescence-based switch-on detection method of 5-formyluracil in DNA, Nano Research, 2017, 10 (7), 2449−2458.

19.Yafen Wang, Chaoxing Liu, Tianlu Wang, Tingting Hong, Haomiao Su, Shuyi Yu, Hongwei Song, Songmei Liu, Xin Zhou, Wuxiang Mao* and Xiang Zhou*, Highly Selective Detection of 5-Methylcytosine in Genomic DNA Based on Asymmetric PCR and Specific DNA Damaging Reagents, Analytical Chemistry, 2016, 88 (6), 3348−3353.