EDUCATION AND EMPLOYMENT
College of Chemistry and Molecular Sciences, Wuhan University, B.S., 1998-2002
College of Chemistry and Molecular Sciences, Wuhan University, Ph.D., 2002-2007, Organic Chemistry, supervisor Dr. Xiang Zhou
College of Chemistry and Molecular Sciences, Wuhan University, Lecturer, 2007-2010
College of Chemistry and Molecular Sciences, Wuhan University, A.P., 2010-2018
University of Chicago, Postdoc and Visiting scholar, 2013-2016, supervisor Dr. Chuan He
College of Chemistry and Molecular Sciences, Wuhan University, Prof., 2018-
RESEARCH FIELD
Based on chemical probes combined with Molecular Biology, Genomics, Cell Biology and other means, we develop new techniques to research nucleic acid epigenetic modification, nucleic acid structure, and nucleic acid/protein interaction. Functional studies and the application in clinical disease diagnosis are explored.
AWARDS AND HONORS
1. The First Prize of Natural Science of Hubei Science and Technology Award (Molecular Recognition and Regulation of nucleic acids, 2nd author), 2020
2. Supported by National Science Fund for Excellent Young Scholars, National Natural Science Foundation of China, 2018
3. Chutian Scholar, Hubei Province Chutian Scholar Program, 2015
4. The First Prize of Natural Science of Hubei Science and Technology Award (Design, synthesis and biological activity studies of small molecules targeting nucleic acids, 2nd author), 2010
5. The First Prize, The fifth Young Teachers' Teaching Competition, Wuhan University, 2010
6. Supported by "Young Chemist Award", the Royal Socirty of Chemistry, 2009
7. Host 4 General Programs, 1 Youth Fund, the National Natural Science Foundation of China. And Key Program: Mechanisms of regulatory action of non-coding RNAs in the process of gene information transmission
8. Has published more than 80 papers in SCI, and more than 30 papers in Nat. Chem. Biol., Cancer Cell, Nat. Commun., J. Am. Chem. Soc., Angew. Chem. Int. Ed as co-first/corresponding author
PUBLICATIONS
1.Ruitu Lyu, Tong Wu, Allen C. Zhu, Diana C. West-Szymanski, Xiaocheng Weng, Mengjie Chen, Chuan He*. KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal-assisted labeling. Nature Protocols, 2021, accepted.
2.Yafen Wang, Shaoqing Han, Ruoxi Ran, Anling Li, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Zhigang Zhao, Shihui Song, Xiaocheng Weng*, Songmei Liu*, Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome. Nature Communications 2021, 12, 5629.
3.Wang, Y. F.; Zhang, X.; Han, S. Q.; Yang, W.; Chen, Z. G.; Wu, F.; Liu, J. Z.; Weng, X. C.*; Zhou, X.*, Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase. Acs Central Science 2021, 7 (6), 973-979. (封面文章)
4.Wang, Q.; Tan, K. Y.; Wang, H.; Shang, J. H.; Wan, Y. Q.; Liu, X. Q.; Weng, X. C.; Wang, F. A.*, Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation. Journal of the American Chemical Society 2021, 143 (18), 6895-6904.
5. Wang, B. Y.; Zhang, X.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, One-pot fluorescent assay for sensitive detection of APOBEC3A activity. RSC Chemical Biology 2021, 2 (4), 1201-1205.
6.Wang, B. Y.; Chen, Y.; Zhang, X.; Jiang, Z. R.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA. Chemical Communications 2021, 57 (22), 2784-2787.
7.Li, W.; Yang, H. M.; Wang, Y. F.; Weng, X. C.*; Wang, F.*, Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch. Analyst 2021, 146 (14), 4450-4453.
8.Huang, J. G.; Zhao, R. Q.; Mo, J.; Wang, F.; Weng, X. C.*; Zhou, X. *, N-3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling. Chembiochem 2021, 22 (9), 1559-1562.
9.Zhang, Y. J.; Zhang, Q. Y.; Weng, X. C.; Du, Y. H.*; Zhou, X., NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA. Analytica Chimica Acta 2021, 1145, 52-58.
10.Huang, J. G.; Zhao, R. Q.; Qin, S. S.; Yang, S. X.;Li, W.; Mo, J.; Wang, F.; Du, Y. H.; Weng, X. C.*; Zhou, X., 4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA. Chembiochem 2021, 22, 212-216.
11.Mo, J.; Chen, Z. G.; Qin, S. S.; Li, S.; Liu, C. G.; Zhang, L.; Ran, R. X.; Kong, Y.; Wang, F.; Liu, S. M.; Zhou, Y.; Zhang, X. L.; Weng, X. C.*; Zhou, X.*, TRADES: Targeted RNA Demethylation by SunTag System. Advanced Science 2020, 7 (19), 202001402.
12.Chen, Yuqi; Wu, Fan; Chen, Zonggui; He, Zhiyong; Wei, Qi; Zeng, Weiwu; Chen, Kun; Xiao, Feng; Yuan, Yushu; Weng, Xiaocheng; Zhou, Yu; Zhou, Xiang*, Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics. Advanced Science 2020, 7 (8), 1900997.
13.Weng, X.; Gong, J.; Chen, Y.; Wu, T.; Wang, F.; Yang, S.; Yuan, Y.; Luo, G.; Chen, K.; Hu, L.; Ma, H.; Wang, P.; Zhang, Q. C.*; Zhou, X.*; He, C.*, Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical Biology 2020, 16 (5), 489-492.
14.Yang, S.; Wu, F.; Peng, S.; Wang, F.; Chen, Y.; Yuan, Y.; Weng, X.*; Zhou, X., A m6A sensing method by its impact on the stability of RNA double helix. Chemistry undefinedamp; Biodiversity 2020, https://doi.org/10.1002/cbdv.202000050.
15.Liu, Y. Q.; Zhang, X.; Liu, M.; Xu, F.; Zhang, Q. Y.; Zhang, Y. J.; Weng, X. C.; Liu, S. M.; Du, Y. H.*; Zhou, X.*, Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification. Analytica Chimica Acta 2020, 1101, 169-175.
16.Zou, G. R.; Liu, C. X.; Fang, Z. T.; Jia, S. K.; Yang, W.; Zhang, X.; Chen, M. K.; Tian, T.; Weng, X. C.; Zhou, X.*, Photocaged probes for spatiotemporal imaging. Sensors and Actuators B-Chemical 2019, 288, 113-119.
17.Li, W.; Zeng, W. W.; Chen, Y.; Wang, F.; Wu, F.; Weng, X. C.*; Zhou, X.*, Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity. Analyst 2019, 144 (15), 4472-4476.
18.Li, W.; Wang, F.; Chen, Y.; Weng, X. C.*; Zhou, X., A sensitive and radiolabeling-free method for pseudouridine detection. Analytical Biochemistry 2019, 581.
19.Chen, Y.; Yang, S.; Peng, S.; Li, W.; Wu, F.; Yao, Q.; Wang, F.; Weng, X.*; Zhou, X.*, N1-methyladenosine detection with CRISPR-Cas13a/C2c2. Chemical Science 2019, 10, 2975-2979
20.Zhong, X.; Yu, J. Y.; Frazier, K.; Weng, X. C.; Li, Y.; Cham, C. M.; Dolan, K.; Zhu, X. R.; Hubert, N.; Tao, Y.; Lin, F. F.; Martinez-Guryn, K.; Huang, Y.; Wang, T.; Liu, J. Z.; He, C.*; Chang, E. B.*; Leone, V.*, Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m(6)A mRNA Methylation. Cell Reports 2018, 25 (7), 1816-+.
21.Yuan, Y. S.; Hong, T. T.; Chen, Y.; Wang, Y. F.; Qiu, X. P.; Zheng, F.; Weng, X. C.*; Zhou, X.*, Luminescence Sensing for Qualitative and Quantitative Detection of 5-Methylcytosine. Analytical Chemistry 2018, 90 (16), 10064-10068.
22.Wu, F.; Liu, W. T.; Yang, S. X.; Yao, Q.; Chen, Y.; Weng, X. C.*; Zhou, X.*, An aptamer-based ligation-triggered rolling circle amplification strategy for ATP detection and imaging in situ. Journal of Photochemistry and Photobiology a-Chemistry 2018, 355, 114-119.
23.Wang, Y. F.; Liu, C. X.; Zhang, X.; Yang, W.; Wu, F.; Zou, G. R.; Weng, X. C.*; Zhou, X.*, Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction. Chemical Science 2018, 9 (15), 3723-3728.
24.Wang, Y. F.; Liu, C. X.; Wu, F.; Zhang, X.; Liu, S.; Chen, Z. G.; Zeng, W. W.; Yang, W.; Zhang, X. L.; Zhou, Y.; Weng, X. C.; Wu, Z. G.; Zhou, X.*, Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe. Iscience 2018, 9, 423-+.
25.Su, R.; Dong, L.; Li, C.; Nachtergaele, S.; Wunderlich, M.; Qing, Y.; Deng, X.; Wang, Y.; Weng, X.; Hu, C.; Yu, M.; Skibbe, J.; Dai, Q.; Zou, D.; Wu, T.; Yu, K.; Weng, H.; Huang, H.; Ferchen, K.; Qin, X.; Zhang, B.; Qi, J.; Sasaki, A. T.; Plas, D. R.; Bradner, J. E.; Wei, M.; Marcucci, G.; Jiang, X.; Mulloy, J. C.; Jin, J.*; He, C.*; Chen, J.*, R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA Signaling. Cell 2018, 172 (1-2), 90-+.
26.Luo, G. Z.; Hao, Z. Y.; Luo, L. Z.; Shen, M. R.; Sparvoli, D.; Zheng, Y. Q.; Zhang, Z. J.; Weng, X. C.; Chen, K.; Cui, Q.; Turkewitz, A. P.; He, C.*, N-6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA. Genome Biology 2018, 19.
27.Hong, T. T.; Yuan, Y. S.; Chen, Z. G.; Xi, K.; Wang, T. L.; Xie, Y. L.; He, Z. Y.; Su, H. M.; Zhou, Y.; Tan, Z. J.; Weng, X. C.*; Zhou, X.*, Precise Antibody -Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution. Journal of the American Chemical Society 2018, 140 (18), 5886-5889.
28.Liu, C.; Wang, Y.; Yang, W.; Wu, F.; Zeng, W.; Chen, Z.; Huang, J.; Zou, G.; Zhang, X.; Wang, S.; Weng, X.; Wu, Z.; Zhou, Y.; Zhou, X.*, Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA. Chemical Science 2017, 8 (11), 7443-7447.
29.Li, Z.; Weng, H.; Su, R.; Weng, X.; Zuo, Z.; Li, C.; Huang, H.; Nachtergaele, S.; Dong, L.; Hu, C.; Qin, X.; Tang, L.; Wang, Y.; Hong, G.-M.; Huang, H.; Wang, X.; Chen, P.; Gurbuxani, S.; Arnovitz, S.; Li, Y.; Li, S.; Strong, J.; Neilly, M. B.; Larson, R. A.; Jiang, X.; Zhang, P.; Jin, J.*; He, C.*; Chen, J.*, FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N-6-Methyladenosine RNA Demethylase. Cancer Cell 2017, 31 (1), 127-141.
